| 酸性α-淀粉酶的分离纯化与酶学性质研究 |
| |
| 引用本文： | 胡元森,潘涛,李翠香. 酸性α-淀粉酶的分离纯化与酶学性质研究[J]. 生物技术通报, 2010, 0(3) |
| |
| 作者姓名： | 胡元森 潘涛 李翠香 |
| |
| 作者单位： | 河南工业大学生物工程学院,河南工业大学粮油重点实验室与工程中心,郑州,450052 |
| |
| 基金项目： | 河南工业大学博士基金项目 |
| |
| 摘 要： | 纯化了枯草芽胞杆菌xm-1菌株酸性α-淀粉酶,并对其酶学性质进行了研究。通过硫酸铵沉淀和Sephadex G-75凝胶层析将酸性α-淀粉酶粗酶液纯化了32.5倍,活力回收率为10.0%。酶性质测定结果表明,该酸性α-淀粉酶分子量约为60kD,最适反应温度为45℃、最适作用pH5.0,该酶在pH3.4-6.0下稳定,高温耐受性差。Cu2+、Zn2+、EDTA对酶有不同程度的抑制作用,Ca2+和Mn2+对酶具有较强的激活作用。
|
| 关 键 词： | 酸性α-淀粉酶 蛋白纯化 酶学性质 Acid-stable α-amylase |
Purification and Properties of an Acid-stable Alpha-amylase from Bacillus subtilis |
| |
| Hu Yuansen,Pan Tao,Li Cuixiang. Purification and Properties of an Acid-stable Alpha-amylase from Bacillus subtilis[J]. Biotechnology Bulletin, 2010, 0(3) |
| |
| Authors: | Hu Yuansen Pan Tao Li Cuixiang |
| |
| Affiliation: | Hu Yuansen Pan Tao Li Cuixiang(Bioengineering College,Henan University of Technology,Key Lab , Engineering Centre of Food , Oil in Henan University of Technology,Zhengzhou 450052) |
| |
| Abstract: | An acid-stable α-amylase produced by Bacillus subtilis xm-1 was purified and the properties was subsequently measured.The crude amylase was purified 32.5 times with 10.0% recovery of enzyme activity through ammonium sulfate precipitation and SephadexG-75 gel chromatography.The enzyme proerties showed that the molecular weight of the purified α-amylase was approximate 60 kD,and the optimum pH and temperature was 5 0 and 45℃,respectively.Metal ion of Cu~(2+),Zn~(2+),EDTA inhibited α-amylase activity at various extent,while the Ca~(2+)and Mn~(2+) distinctly enhanced the activity. |
| |
| Keywords: | Protein purification Enzymatic properties |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
