Enhanced production of harringtonine and homoharringtonine in Cephalotaxus fortunei callus culture by periodic temperature oscillation |
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Authors: | Zhang Wei Bai Xuefang Bu Zongshi Wang Jinmei Yu Xingju Yuan Quan |
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Institution: | (1) Department of Biochemical Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, People's Republic of China;(2) Department of Chemical Engineering, The University of Adelaide, Adelaide, Australia |
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Abstract: | Total production of harringtonine, homoharringtonine and isoharringtonine in solid cultures of Cephalotaxus fortunei was 1.22 mg/l by periodic oscillation in temperature between 10 and 25°C every 12 h for 45 days. Production was enhanced 1.8 and 1.3-fold compared to the controls at constant temperatures of 10 or 25°C. For suspension cultures subjected to such an oscillation every 24 h for 30 days, total alkaloid production of 0.18 mg/l was achieved, a 2.0 and 1.1-fold improvement compared to the suspension culture controls, correspondingly. |
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