Quantification of tipranavir in human plasma by high-performance liquid chromatography with UV detection |
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Authors: | Giraud Emmanuelle Rey Elisabeth Tréluyer Jean-Marc Pons Gérard Jullien Vincent |
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Affiliation: | Université Paris-Descartes, Faculté de Médecine, Assistance Publique-H?pitaux de Paris, Groupe Hospitalier Cochin-Saint-Vincent de Paul, Pharmacologie Clinique, France. |
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Abstract: | A simple method for the quantification of tipranavir, a new non-peptidic protease-inhibitor, was developed. An internal standard, prazepam, was added to 100 microl of plasma before a liquid-liquid extraction by 3 ml of tert-butyl methyl ether. The extracts were evaporated to dryness and reconstituted with 100 microl of mobile phase before being injected in the chromatographic system. The separation was made on a C8 column using sodium acetate buffer (pH 5):methanol:acetonitrile (35:30:35, v/v/v) as mobile phase. The detection was performed at a wavelength of 260 nm. The method was linear and has been validated over a concentration range of 2-80 mg/l. The mean precision and accuracy of the method were respectively, 10.5 and -9.1%. The mean recovery was 70.8%. |
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