Phosphorylation of F1F0 ATPase δ-Subunit Is Regulated by Platelet-Derived Growth Factor in Mouse Cortical Neurons In Vitro |
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| Authors: | Frank X Zhang †Weiying Pan ‡ James B Hutchins |
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| Institution: | Departments of Anatomy and; Neurology, University of Mississippi Medical Center, Jackson, Mississippi;and; Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland, U.S.A. |
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| Abstract: | Abstract: The δ subunit of F1F0 ATPase (ATP synthase complex) is part of the stalk connecting the F1 and F0 moieties. Studies in Escherichia coli suggest that the analogous bacterial subunit, called ε, is essential for the ATPase assembly energy coupling. Platelet-derived growth factor (PDGF) is an important growth factor for various cell types, including neurons of the CNS. Using two-dimensional gel electrophoresis, microsequencing, western blot analysis, and immunoprecipitation techniques, we have found that PDGF induces phosphorylation of the δ subunit or a closely related peptide in cultured mouse cortical neurons. |
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| Keywords: | Growth factor Mitochondrion ATP synthase CNS |
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