Identification and mapping of polymorphic RAPD markers of pea (Pisum sativum L.) genome |
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| Authors: | Koveza O V Kokaeva Z G Konovalov F A Gostimsky S A |
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| Institution: | Department of Genetics, Lomonosov Moscow State University, Moscow, 119899 Russia. kovezaO@mail.ru |
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| Abstract: | Various pea cultivars, lines, and mutants were studied by the RAPD method. Polymorphic fragments characteristic of certain pea genotypes and which can be used for identifying genotypes were detected. Inheritance of some polymorphic RAPD fragments was studied. Mendelian inheritance of these fragments was shown. By analyzing the data obtained in studies of RAPD polymorphism, genetic distances between different pea cultivars, lines, and mutants were calculated and a genealogic dendogram showing a varying extent of differences between RAPD patterns was constructed. Ten new RAPD markers linked to various pea genes were detected. Genetic distances between RAPD markers and genes to which they are linked were calculated, and the respective disposition of RAPD markers on chromosomes was established. |
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