Cloning of a polyubiquitin gene fromNicotiana tabacum and comparison to other polyubiquitin genes |
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Authors: | Jin Hyun Kim Soo Min Park Ho Rim Lee Jae-Ho Pyee Choo Bong Hong Kwang Woong Lee |
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Institution: | (1) Department of Biology, Seoul National University, 151-742 Seoul, Korea;(2) Institute for Molecular Biology and Genetics, Seoul National University, 151-742 Seoul, Korea;(3) Department of Molecular Biology, Dankook University, 140-714 Seoul, Korea |
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Abstract: | Using a tobacco cDNA clone as a probe, a genomic clone named TUQG-4, coding for a tobacco polyubiquitin protein with the five
head-to-tail repeats of ubiquitin monomer was isolated. The five ubiquitin units were completely conserved except for the
extra phenylalanine at the carboxy terminus of the last ubiquitin monomer. The putative open reading frame identified from
the nucleotide sequence showed two possible intron sequences in the coding region for the first ubiquitin monomer. When the
amino acid sequence deduced from the nucleotide sequence of TUQG-4 was compared to the amino acid sequences coded by other
polyubiquitin genes of tobacco, there were three or four amino acid differences in the sequence. When the nucleotide sequences
coding for the ubiquitin monomers were compared for various species origins, the degree of identity was at the highest between
the ubiquitin monomers in one polyubiquitin and did not reflect the distance of the phylogenetic relationship. |
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Keywords: | genomic clone Nicotiana tabacum nucleotide sequence comparison polyubiquitin |
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