Improved batch culture of Lactobacillus helveticus with reused cells reactivated at acidic pH: the minimal volume of reused culture fraction |
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Authors: | A. Amrane |
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Affiliation: | (1) Laboratoire des Procédés de Séparation (UPRES EA3191, UC INRA), Université de Rennes 1, Campus de Beaulieu, Bât. 10 A, CS 74205, 35042 Rennes Cedex, France |
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Abstract: | Three modes of batch culture of Lactobacillus helveticus were compared: the conventional culture inoculated with 11% (v/v) seed culture and cultures inoculated with 0.1 or 11% (v/v) of a volume fraction of the preceding culture, reactivated first at acidic pH. Culture reuse was useful with low nitrogen supplementation of culture medium (5 g yeast extract l–1). Even when the volume of reused culture fraction was minimal (0.1% v/v), a 14% increase in the mean production rate was observed, compared to the conventional mode of batch culture. |
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Keywords: | batch mode cell reuse lactic acid bacteria nitrogen supplementation production rate |
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