Relation between the optimum pH of protein immobilization and its isoelectric points

Data available in literature concerning pH-dependence of immobilization of certain proteins are analyzed. A conclusion is drawn that an optimal pH of the enzyme binding with the matrix surface is determined by properties of the enzymes themselves rather than by the matrix origin. Linear dependence between the pH-optimum of immobilization and the value of their isoelectric points is shown on 19 proteins.