A new member of the CAB gene family: structure,expression and chromosomal location of Cab-8, the tomato gene encoding the Type III chlorophyll a/b-binding polypeptide of photosystem I |
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Authors: | Eran Pickersky Thomas G. Brock Duc Nguyen Neil E. Hoffman Birgit Piechulla Steven D. Tanksley Beverley R. Green |
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Affiliation: | (1) Biology Department, University of Michigan, 48109 Ann Arbor, MI, USA;(2) Department of Plant Biology, Carnegie Institute of Washington, 290 Panama Street, 94305 Stanford, CA, USA;(3) Institut für Biochemie der Pflanze der Universität Göttingen, Untere Karspule 2, 34 Göttingen, FRG;(4) Department of Plant Breeding and Biometry, Cornell University, 14853 Ithaca, NY, USA;(5) Department of Botany, University of British Columbia, V6T 2B1 British Columbia, Vancouver, Canada |
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Abstract: | We have previously reported the isolation and characterization of tomato nuclear genes encoding two types of chlorophyll a/b-binding (CAB) polypeptides localized in photosystem (PS) I and two types of CAB polypeptides localized in PSII. Sequence comparisons shows that all these genes are related to each other and thus belong to a single gene family. Here we report the isolation and characterization of an additional member of the tomato CAB gene family, the single tomato nuclear gene, designated Cab-8, which encodes a third type of CAB polypeptide localized in PSI. The protein encoded by Cab-8 is 65% and 60% divergent from the PSI Type I and Type II CAB polypeptides, respectively. The latter two are 65% divergent from each other. Only some short regions of the polypeptides are strongly conserved. The Cab-8 locus maps to chromosome 10, 9 map units from Cab-7, the gene encoding the Type II PSI CAB polypeptide. The Cab-8 gene contains two introns; the first intron matches in position the single intron in the Type II PSII CAB genes and the second intron matches in position the second intron in the Type II PSI CAB gene. Like other CAB genes, Cab-8 is light-regulated and is highly expressed in the leaf and to a lesser extent in other green organs. |
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Keywords: | chloroplast protein import DNA sequences Lycopersicon esculentum plant evolution RFLP markers |
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