Phosphorylation of brain muscarinic receptor: evidence of receptor regulation |
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| Authors: | A K Ho Q L Ling R Duffield P H Lam J H Wang |
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| Affiliation: | 2. Department of Basic Sciences, College of Medicine at Peoria and Urbana, University of Illinois, Peoria, IL 61605 USA;1. Department of Pharmacology, College of Medicine at Peoria and Urbana, University of Illinois, Peoria, IL 61605 USA;2. Department of Medicine, University of Manitoba, Winnepeg, Canada;1. Department of Pharmacology, University of Manitoba, Winnepeg, Canada;112. Department of Biochemistry, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada |
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| Abstract: | Muscarinic receptor, from porcine synaptic membrane, was purified by affinity chromatography. Molecular weight analysis by SDS-gel electrophoresis revealed one major peptide with an apparent Mr of 68 +/- 2 Kda. The purified receptor was phosphorylated by the catalytic subunit of cAMP-dependent protein kinase resulting in a concomitant loss in specific binding, and this loss was reversed by calcineurin. |
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