In vitro regeneration of Vigna unguiculata (L.) Walp. cv. Blackeye cowpea via shoot organogenesis |
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| Authors: | Jing Qin Mao Mohsin Abbas Zaidi John Thor Arnason Illimar Altosaar |
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| Institution: | (1) Agricultural Biotechnology Laboratories, Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 451 Smyth Road, K1H 8M5, Ottawa, ON, Canada;(2) Biology Department, University of Ottawa, 30 Marie Curie, Station A, P.O. Box 450, K1N 6N5 Ottawa, ON, Canada |
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| Abstract: | An in vitro regeneration system was developed in cowpea Vigna unguiculata (L.) Walp.] Blackeye. Among several explants studied, shoot initiation response was observed from shoot apices of 3–5-day-old seedlings. The optimal medium for maximum shoot initiation comprised MS salts, B5 vitamins, 8.88 μM N
6-benzylaminopurine, 1 gl-1 casein hydrolysate, 342 μM L-glutamine, 3% sucrose, 0.3% phytagel, adjusted to pH 5.8. A shift in pH from 5.8 to 7.0 had no effect on shoot initiation and on number of shoots per explant. The highest shoot initiation frequency (77%) was obtained using this preferred medium, reaching a maximum of eight shoots per explant. For shoot elongation, 14 μM gibberellic acid was supplemented in the shoot initiation medium. Presence of indolebutyric acid in the rooting medium had no effect on root induction. The regenerated plants were fertile and developed normally. |
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| Keywords: | Vigna unguiculata Blackeye cowpea Shoot organogenesis In vitro regeneration |
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