Localization of a symbiotic fix region on Rhizobium meliloti pSym megaplasmid more than 200 kilobases from the nod-nif region |
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| Authors: | J Batut B Terzaghi M Ghérardi M Huguet E Terzaghi A M Garnerone P Boistard and T Huguet |
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| Institution: | (1) Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, CNRS-INRA, Groupement Scientifique Microbiologie Toulouse, BP 27, F-31326 Castanet-Tolosan Cedex, France;(2) Present address: Plant Physiology Division, DSIR, Private Bag, Palmerston North, New Zealand;(3) Present address: Massey University, Palmerston North, New Zealand |
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| Abstract: | Summary We have established the HindIII physical map of a cloned 290 kilobase fragment of the Rhizobium meliloti 2011 pSym megaplasmid. The cloned fragment, which contains nodulation genes as well as the nitrogenase structural genes (nifHDK), has been shown to be colinear with the corresponding genomic region. Using transposon mutagenesis we have demonstrated that a region which is located more than 200 kb from the nifHDK operon on pSym is essential for symbiotic nitrogen fixation.Dedicated to Professor Georg Melchers to celebrate his 50-year association with the journal |
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