Ebolavirus VP35 Coats the Backbone of Double-Stranded RNA for Interferon Antagonism |
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| Authors: | Shridhar Bale Jean-Philippe Julien Zachary A Bornholdt Alexander S Krois Ian A Wilson Erica Ollmann Saphire |
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| Institution: | Department of Immunology and Microbial Sciencea;Department of Integrative Structural and Computational Biologyb;The Skaggs Institute for Chemical Biology,c The Scripps Research Institute, La Jolla, California, USA |
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| Abstract: | Recognition of viral double-stranded RNA (dsRNA) activates interferon production and immune signaling in host cells. Crystal structures of ebolavirus VP35 show that it caps dsRNA ends to prevent sensing by pattern recognition receptors such as RIG-I. In contrast, structures of marburgvirus VP35 show that it primarily coats the dsRNA backbone. Here, we demonstrate that ebolavirus VP35 also coats the dsRNA backbone in solution, although binding to the dsRNA ends probably constitutes the initial binding event. |
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