New Type of Antimicrobial Protein Produced by the Plant Pathogen Clavibacter michiganensis subsp. michiganensis |
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| Authors: | Zhanliang Liu Ping Ma Ingrid Holtsmark Morten Skaugen Vincent G. H. Eijsink May B. Brurberg |
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| Affiliation: | Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norwaya;Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Integrated Pest Management Centre of Hebei Provence, Baoding, Chinab;Norwegian Institute for Agricultural and Environmental Research, Plant Health and Plant Protection Division, Ås, Norwayc |
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| Abstract: | It has previously been shown that the tomato pathogen Clavibacter michiganensis subsp. michiganensis secretes a 14-kDa protein, C. michiganensis subsp. michiganensis AMP-I (CmmAMP-I), that inhibits growth of Clavibacter michiganensis subsp. sepedonicus, the causal agent of bacterial ring rot of potato. Using sequences obtained from tryptic fragments, we have identified the gene encoding CmmAMP-I and we have recombinantly produced the protein with an N-terminal intein tag. The gene sequence showed that CmmAMP-I contains a typical N-terminal signal peptide for Sec-dependent secretion. The recombinant protein was highly active, with 50% growth inhibition (IC50) of approximately 10 pmol, but was not toxic to potato leaves or tubers. CmmAMP-I does not resemble any known protein and thus represents a completely new type of bacteriocin. Due to its high antimicrobial activity and its very narrow inhibitory spectrum, CmmAMP-1 may be of interest in combating potato ring rot disease. |
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