Colorimetric recognition of aromatic amino acid enantiomers by gluconic acid-capped gold nanoparticles |
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Authors: | Yang Jiangxia Li Xiaoqi Du Yingxiang Ma Mingxuan Zhang Liu Zhang Jian Li Peipei |
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Affiliation: | 1.Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, No.24 Tongjiaxiang, Nanjing, 210009, Jiangsu, People’s Republic of China ;2.State Key Laboratory of Natural Medicines, China Pharmaceutical University, No.24 Tongjiaxiang, Nanjing, 210009, Jiangsu, People’s Republic of China ;3.Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, People’s Republic of China ; |
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Abstract: | In this work, we prepared gold nanoparticles (AuNPs) by employing gluconic acid (GlcA) as reducing-cum-stabilizing agent. The proposed GlcA-AuNPs successfully worked as a colorimetric sensor for visual chiral recognition of aromatic amino acid enantiomers, namely tyrosine (d/l-Tyr), phenylalanine (d/l-Phe), and tryptophan (d/l-Trp). After adding L-types to GlcA-AuNPs solution, the color of the mixture changed from red to purple (or gray), while no obvious color change occurred on the addition of D-types. The effect can be detected by naked eyes. The particles have been characterized by transmission electron microscopy, Fourier-transform infrared spectroscopy, zeta potential, the dynamic light scattering analysis as well as UV–Vis spectroscopy. This assay can be used to determine the enantiomeric excess of l-Trp in the range from 0 to + 100%. The method has advantages in simplicity, sensitivity, fast response, and low cost. |
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