Determination of uric acid by an anion-exchange column chromatography |
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| Authors: | D S Hsu S S Chen |
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| Affiliation: | 1. Department of Obstetrics and Gynecology, Long Island Jewish Medical Center, New Hyde Park, New York 11042 USA;2. School of Medicine, Health Science Center, State University of New York at Stony Brook, New York 11794 USA;1. The Australian Wine Research Institute, Waite Precinct, Hartley Grove cnr Paratoo Road, Urrbrae (Adelaide) SA 5064, PO Box 197, Glen Osmond, SA 5064, Australia;2. School of Engineering, University of South Australia, Mawson Lakes Campus, Mawson Lakes, SA 5095, Australia;1. Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand;2. Center of Excellence for Innovation in Analytical Science and Technology, Chiang Mai University, Chiang Mai 50200, Thailand;1. The Australian Wine Research Institute, Waite Precinct, Hartley Grove cnr Paratoo Road, Urrbrae (Adelaide), PO Box 197, Glen Osmond, SA 5064, Australia;2. Future Industries Institute, University of South Australia, Mawson Lakes Campus, Mawson Lakes, SA 5095, Australia;3. Institute of General and Ecological Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland;4. School of Engineering, University of South Australia, Mawson Lakes Campus, Mawson Lakes, SA 5095, Australia;5. Wine Australia, P.O. Box 660, Kent Town, SA 5071, Australia;1. Faculty of Horticulture, University of Agricultural Sciences and Veterinary Medicine “Ion Ionescu de la Brad” Iasi, Romania;2. Agricultural Chemistry Department. Building Marie Curie. Campus of Rabanales. Agrifood Campus of International Excellence ceiA3, University of Córdoba, 14014 Córdoba, Spain |
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| Abstract: | A column chromatography using a conventional anion-exchange resin for the separation of uric acid from other purine metabolites is described. It uses a HCl gradient, and the amount of uric acid is quantified directly by monitoring the absorbance of the effluent at 285 nm. The linear range of response is 0.5 to 100 nmol. The method was applied to the analysis of uric acid in urine and serum. Urine was injected directly into the system, while serum required removal of an interfering substance which absorbs the light and coelutes with uric acid. However, this substance was simply removed by heat coagulation of serum by heating in a boiling water bath for 2 min. |
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