Detection of siderophores in growing cultures ofPseudomonas spp. |
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| Authors: | L Raaska L Viikari T Mattila-Sandholm DVM PhD |
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| Institution: | (1) Food Research Laboratory, VTT, Espoo, Finland;(2) Biotechnical Laboratory, VTT, Espoo, Finland;(3) Food Research Laboratory, Technical Research Centre of Finland, P.O. Box 203, SF-02151 Espoo, Finland |
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| Abstract: | Summary The siderophores produced byPseudomonas fluorescens andP. chlororaphis were detected from the culture supernatants in MM9 and modified King's medium by the universal CAS assay at wavelengths 620–690 nm. The CAS assay was applied to detectPseudomonas siderophores directly in situ, during their production phase, in modified King's medium. Optimum results were detected with a final CAS concentration of 0.025 mM and an iron concentration of 1.25  M. The problems of the method are discussed with respect to the absorbance spectrum, the toxicity of the HDTMA detergent, the influence of the iron concentration and the complexity of media for siderophore production. |
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| Keywords: | CAS-assay Iron-binding Pseudomonas Siderophore |
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