| 一种改良的鱼类染色体富集制片方法 |
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| 引用本文： | 海燕,黄晓,易梅生,余其兴HAI Yan,HUANG Xiao,YI Mei-sheng,YU Qi-xing.一种改良的鱼类染色体富集制片方法[J].遗传,2001,23(2):151-233. |
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| 作者姓名： | 海燕 黄晓 易梅生 余其兴HAI Yan HUANG Xiao YI Mei-sheng YU Qi-xing |
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| 作者单位： | 武汉大学生命科学学院生物学系，湖北武汉 430072
Department of Biology, School of Life Science, Wuhan University, Wuhan 430072, China |
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| 摘 要： | 运用淋巴细胞分离液处理黄鳝肾脏细胞悬浮液后，成功地分离了其中红细胞和淋巴细胞。以富集的淋巴细胞进行染色体制片，可避免有核红细胞的影响，获得高分裂指数的黄鳝染色体标本。该方法简便、结果稳定,亦适用于其他鱼类、两栖类和鸟类的染色体制片。
Abstract：After treated by the lymphocyte separation medium, the erythrocytes and lymphocytes were separated successfully in the kidney cells suspension of rice field eel. Using the enriched lymphocytes to prepare chromosome, we get the high split-index chromosome preparations without the interference of the erythrocytes. This technique is simple and convenient to prepare the chromosome of other fishes or other animals for FISH.
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| 关 键 词： | 淋巴细胞分离 黄鳝 rice field eel 染色体制片 Keywords separation of lymphocytes |
An Improved Technique of Enriched Fish Chromosome Preparations |
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| HAI Yan,HUANG Xiao,YI Mei-sheng,YU Qi-xing.An Improved Technique of Enriched Fish Chromosome Preparations[J].Hereditas,2001,23(2):151-233. |
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| Authors: | HAI Yan HUANG Xiao YI Mei-sheng YU Qi-xing |
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