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Surface components of HeLa cells that inhibit cytadherence of Chlamydia trachomatis
Authors:Theresa D Joseph  Subir K Bose
Institution:Department of Microbiology, St. Louis University School of Medicine, St. Louis, Missouri, U.S.A.
Abstract:Abstract Isolated HeLa plasma membrane (PM) preparations and extracts containing either cell-surface proteins or lipids were examined for inhibition of adherence of radiolabeled Chlamydia trachomatis serovar E elementary bodies to glutaraldehydefixed HeLa monolayers. A dose-dependent adherence-inhibitory activity could be demonstrated with the PM. A urea extract as well as lipids from HeLa cells also inhibited chlamydial cytadherence. The inhibitory activity of the PM was trypsin-sensitive. It was absent when the urea extract was prepared from trypsin-treated HeLa cells. The urea extract was subjected to electrophoresis and protein blotting using a native gel system. Probing with radiolabeled chlamydial cytadhesin showed a single protein present in the urea extract that could represent a HeLa cell protein receptor for the chlamydiae.
Keywords:Chlamydia trachomatis            HeLa cells  Lipids  Protein receptor
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