Effect of superoxide dismutase,allopurinol and glucocorticoids on liver and lung metallothionein induction by endotoxin in the rat |
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Authors: | Mercedes Giralt Angeles Blanquez Javier Avila Juan Hidalgo |
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Affiliation: | (1) Departamento de Biología Celular y Fisiología, Unidad de Fisiología Animal, Facultad de Ciencias, Universidad Autónoma de Barcelona, Bellaterra, Spain;(2) Servicio de Medicina Interna, Hospital de Bellvitge Prínceps d'Espanya, Hospitalet de Llobregat, Barcelona, Spain;(3) Departmento de Biología Celular y Fisiología, Unidad Fisiología Animal, Facultad de Ciencias, Universidad Autónoma de Barcelona, 08193 Bellaterra, Barcelona, Spain |
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Abstract: | Liver and lung metallothionein (MT) levels were increased by endotoxin. The administration of superoxide dismutase (SOD) or allopurinol (ALLO) before (30–60 min) or after (24–32 h) the endotoxin treatment either increased or did not affect the effect of endotoxin on MT levels, depending on the particular treatment and tissue. SOD and ALLO also increased liver and lung MT levels in control rats. In contrast, liver MT levels tended to be decreased by the glucocorticoid prednisolone (PRED) when administered before the endotoxin and were significantly decreased when it was administered after endotoxin. The effect of PRED on lung MT levels was completely different, since it decreased the effect of endotoxin when injected before the lipopolysaccharide, but increased it when injected after the endotoxin. Liver lipid peroxidation, as measured by thiobarbituric acid reactants (TBARs), increased after endotoxin in the liver but not in the lung, an effect even potentiated in some cases by the antioxidants studied. As expected, tissue MT and TBARs could not be correlated. |
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Keywords: | allopurinol glucocorticoids lipid peroxidation metallothionein prednisolone superoxide dismutase |
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