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Luminescent ruthenium(II) amidodipyridoquinoxaline biotin complexes that display higher avidin-induced emission enhancement
Authors:Kenneth Kam-Wing Lo  Terence Kwok-Ming Lee
Institution:Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, PR China
Abstract:A series of luminescent ruthenium(II) amidodipyridoquinoxaline biotin (dpq-B) complexes Ru(N-N)2(N-N′)](PF6)2 (N-N = 2,2′-bipyridine (bpy), 1,10-phenanthroline (phen), 4,7-diphenyl-1,10-phenanthroline (Ph2-phen); N-N′ = 2-((2-biotinamido)ethyl)amidodipyrido3,2-f:2′,3′-h]quinoxaline (dpq-C2-B), 2-((6-biotinamido)hexyl)amidodipyrido3,2-f:2′,3′-h]quinoxaline (dpq-C6-B)) has been designed as new luminescent probes for avidin. The electrochemical and photophysical properties of these complexes have been investigated. Upon irradiation, all the complexes exhibited metal-to-ligand charge-transfer (3MLCT) (dπ(Ru) → π(diimine)) emission in fluid solutions at 298 K and in low-temperature glass. In aqueous buffer, the emission was extremely weak, probably a consequence of hydrogen-bonding interactions between the amide moiety of the dpq-B ligands and the water molecules. The avidin-binding properties of all the complexes have been studied by 4′-hydroxyazobenzene-2-carboxylic acid (HABA) assays, luminescence titrations, kinetics experiments and confocal microscopy using avidin-conjugated microspheres.
Keywords:Avidin  Biotin  Luminescence  Probes  Ruthenium complexes
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