Regulation of the expression of low affinity GABAA receptors in rat cerebellar granule cells |
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Authors: | Luccardini C Barilà B Cupello A Robello M Mainardi P |
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Institution: | (1) Centro di Neurofisiologia Cerebrale, C.N.R., Genova,, IT;(2) INFM and Dipartimento di Fisica, Università di Genova, and, IT;(3) Dipartimento di Scienze Neurologiche, Università di Genova, Italy, IT |
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Abstract: | Summary. GABAA receptors of cerebellar granule cells obtained from neonatal rats and kept in culture were studied by labelled muscimol binding.
The data show that, according to the maturational state of those cells in vivo, one or two binding components appear. The
low affinity component seems to be the one appearing later. The expression of this component seems to be regulated by protein
tyrosine phosphorylation. In fact, its expression is down regulated by the protein tyrosine kinase (PTK) inhibitor, genistein.
Viceversa, its expression is upregulated by insulin like growth factor I (IGF-I), most probably via PTK activation.
A possible interpretation of the data is that in vivo IGF-I is one of the endogenous messages leading to the expression of
this component during development. Another endogenous factor involved may be GABA itself.
Low affinity GABAA receptors appear to be the ones involved in inhibitory synaptic transmission at glomeruli. Whereas the high affinity ones
probably correspond to extrasynaptic GABAA receptors mediating the tonic form of inhibition in cerebellar granules.
Received December 12, 2000 Accepted February 12, 2001 |
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Keywords: | : Amino acids Cerebellar granules Development Cells cultures GABAA receptors Muscimol binding PTK |
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