Failure of multinucleated giant cell formation in k562 cells infected with newcastle disease virus and human parainfluenza type 2 virus |
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Authors: | Yamakawa Izumi Tsurudome Masato Kawano Mitsuo Nishio Machiko Komada Hiroshi Ito Morihiro Uji Yukitaka Ito Yasuhiko |
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Institution: | Department of Microbiology, Mie University Graduate School of Medicine, Japan. i-yama28@clin.medic.mie-u.ac.jp |
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Abstract: | When K562 cells were infected with Newcastle disease virus (NDV) or human parainfluenza type 2 virus (hPIV-2), polykaryocyte formation could not be detected. Failure of multinucleated giant cell formation in K562 cells infected with either NDV or hPIV-2 is due to disturbance of the viral envelope-cell fusion step or to defect in the cell-cell fusion step, respectively. Especially, NDV completely replicated in K562 cells, and the hemagglutinin-neuraminidase and fusion proteins expressed on the cell surface of NDV-infected K562 cell were fully functional for fusion inducing activity. Therefore, the cell membranes of K562 cells are considered to be resistant to virus-induced cell fusion. Membrane fusion is regulated by many host factors including membrane fluidity, cytoskeletal systems, and fusion regulatory proteins system. An unknown regulatory mechanism of virus-induced cell fusion may function on the cell surface of K562 cells. |
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Keywords: | newcastle disease virus human parainfluenza type 2 virus fusion K562 cells |
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