Emesis of rhesus monkeys induced by intragastric administration with the HEp-2 vacuolation factor (cereulide) produced by Bacillus cereus |
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| Authors: | KunihiroShinagawa HirotakaKonuma HiroyukiSekita ShunjiSugii |
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| Institution: | Faculty of Agriculture, Department of Veterinary Medicine, Iwate University, 3-18-8, Ueda, Morioka, Iwate 020, Japan; National Institute of Hygienic Sciences, Kami-Yoga, Setagaya, Tokyo 158, Japan; University of Osaka Prefecture, Gakuen-cho, Sakai, Osaka 593, Japan |
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| Abstract: | Abstract To study the correlation between emetic toxin and HEp-2 vacuole activity produced by Bacillus cereus isolated from an outbreak of vomiting-type food poisoning, some properties and emetic activities of both purified HEp-2 factor (cereulide) and partially purified factor to rhesus monkeys were determined. The results indicate that both cereulide and partially purified factor were very stable to digestion with proteolytic enzymes, different pH, and heating. Vomiting was induced in the rhesus monkeys orally administered with both substances. From these findings, cereulide (or HEp-2 vacuole factor) is strongly suggested to be an emetic toxin itself. |
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| Keywords: | Bacillus cereus Emesis HEp-2 cells Rhesus monkey Vacuolation factor |
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