Immunohistochemical localization of heparin-binding epidermal growth factor-like growth factor in normal skin and skin cancers |
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Authors: | Marc T Downing David R Brigstock Mark H Luquette Missy Crissman-Combs Gail E Besner |
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Institution: | (1) Department of Surgery, The Ohio State University and Children's Hospital Columbus, U.S.A;(2) Pathology, The Ohio State University and Children's Hospital Columbus, U.S.A |
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Abstract: | Summary Heparin-binding epidermal growth factor (EGF)-like growth factor is a 22-kDa glycoprotein that was originally identified as
a secreted product of cultured human macrophages. Although the growth factor mRNA has been identified in various cells and
tissues, the tissue distribution of the protein itself has rarely been demonstrated. In this study, the EGF-like growth factor
was detected immunohistochemically in a variety of human skin samples by indirect immunofluorescence using a polyclonal rabbit
antiserum raised against residues 26–41 of mature heparin-binding EGF. The keratinocytes of a variety of epithelium-derived
structures demonstrated reproducible, specific staining for the EGF. In normal tissues, this staining was prominent in the
basal cells of the epidermis and in the epithelial cells lining epidermal appendages such as hair follicles, sebaceous sweat
glands and eccrine sweat glands. In addition, specific staining was detected in skin cancers derived from the basal epithelial
cell layer, including basal and squamous cell carcinomas of the skin, with no staining detected in melanoma specimens. Immunoreactive
heparin-binding EGF was characteristically associated with the surface of cells. With minor exceptions, the immunoreactive
sites are identical to the known EGF receptor distribution in the skin, and suggest that keratinocyte-derived heparin-binding
EGF may act in concert with other EGF family members in processes such as skin morphogenesis and wound repair, as well as
in the development of skin cancers
This revised version was published online in November 2006 with corrections to the Cover Date. |
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