| RAPD分析在绢丝昆虫亲缘关系研究中的应用：Ⅱ．柞蚕品种间的遗传差异 |
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| 引用本文： | 桂慕燕,左正宏,王学民,陈元霖.RAPD分析在绢丝昆虫亲缘关系研究中的应用：Ⅱ．柞蚕品种间的遗传差异[J].遗传,2001,23(5):452-454. |
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| 作者姓名： | 桂慕燕 左正宏 王学民 陈元霖 |
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| 作者单位： | 厦门大学生命科学学院 |
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| 基金项目： | 国家自然科学基金资助项目部分内容(批准号39870410) |
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| 摘 要： | 采用RAPD技术，对5个柞蚕品种的遗传差异进行比较研究，结果表明，所采用40个随机引物中，有27个引物扩增谱清晰且重复性较好，扩增总片段数253条，单个引物的扩增片段数在4－16之间，片段大小在0．33－3．0kb之间。不同柞蚕品种间的遗传差异较小，遗传距离（D）在0．066－0．1659之间，根据D值，由UPGMA聚类分析软件绘制了它们的分子进化树。
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| 关 键 词： | 柞蚕 RAPD 品种差异 亲缘关系 |
| 文章编号： | 0253-9772(2001)05-0452-03 |
| 修稿时间： | 2000年9月22日 |
Application of RAPD Technique in Genetic Relationship of Silk Insect II. Genetic Variance in Antheraea pernyi |
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| GUI Mu yan,ZUO Zheng hong,WANG Xue min,CHEN Yuan lin.Application of RAPD Technique in Genetic Relationship of Silk Insect II. Genetic Variance in Antheraea pernyi[J].Hereditas,2001,23(5):452-454. |
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| Authors: | GUI Mu yan ZUO Zheng hong WANG Xue min CHEN Yuan lin |
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| Abstract: | Random amplified Polymorphic DNA (RAPD) was used to analyze the genetic diversity among Antheraea pernyi . The genetic variance of five Antheraea pernyi was studied. The result showed that: 27of 40 arbitrary primers could amplify clear and repeating bands. A total of 262 fragments were obtained. Each primer gave 4-16 bands and the average was 9.7. The length of the band was 0.33-3.0kb. The D value between different breeds of Antheraea pernyi was 0.066-0.1659. The D value was used to construct a dendrogram by UPGMA. |
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| Keywords: | Antheraea pernyi RAPD genetic variance |
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