The effect of laser radiations on human ceruloplasmin. Absorption, circular dichroism and electron paramagnetic resonance study. |
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Authors: | M Herve A Garnier L Tosi M Steinbuch |
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Affiliation: | 2. U.E.R. De Medecine et Biologie Humaine, Université Paris XIII, Bobigny, France;3. Department de Fractionnement, Centre National de Transfusion Sanguine, Orsay, France |
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Abstract: | Laser radiations at wavelengths ranging from 514.5 to 360.0 nm decolorize human ceruloplasmin. Kinetic behavior of the two chromophores absorbing at 610 nm, as measured by absorption and circular dichroism data, indicate different quantum yields of the two type I copper ions, whose maximum lies approximatively at 400 nm. Furthermore, as Electron Paramagnetic Resonance measurements demonstrate, the photochemical process involves reduction of the two type I copper ions leaving type II copper unchanged. |
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