| 猪流行性腹泻病毒反向遗传操作技术及其应用 |
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| 引用本文： | 于瑞嵩,董世娟,司伏生,蒋凤英,谢春芳,陈冰清,喻利,李震. 猪流行性腹泻病毒反向遗传操作技术及其应用[J]. 生物工程学报, 2017, 33(2): 205-216 |
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| 作者姓名： | 于瑞嵩 董世娟 司伏生 蒋凤英 谢春芳 陈冰清 喻利 李震 |
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| 作者单位： | 1 上海市农业科学院畜牧兽医研究所，上海 201106；2 上海市农业遗传育种重点实验室，上海 201106,1 上海市农业科学院畜牧兽医研究所，上海 201106；2 上海市农业遗传育种重点实验室，上海 201106,1 上海市农业科学院畜牧兽医研究所，上海 201106,1 上海市农业科学院畜牧兽医研究所，上海 201106；2 上海市农业遗传育种重点实验室，上海 201106,1 上海市农业科学院畜牧兽医研究所，上海 201106；2 上海市农业遗传育种重点实验室，上海 201106,1 上海市农业科学院畜牧兽医研究所，上海 201106,1 上海市农业科学院畜牧兽医研究所，上海 201106,1 上海市农业科学院畜牧兽医研究所，上海 201106；2 上海市农业遗传育种重点实验室，上海 201106 |
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| 基金项目： | 上海市科技支撑项目 (No. 13391901502)，国家自然科学基金(Nos. 31402219, 315725199)，上海市科技兴农重点攻关项目 (沪农科攻字 (2015) 第6-1-9号) 资助。 |
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| 摘 要： | 猪流行性腹泻病毒(PEDV)是引起猪(特别是新生仔猪)急性、高度传染性消化道疾病的主要病原之一;2010年底以来,猪流行性腹泻(PED)的再次暴发给我国乃至全球养猪业造成了巨大经济损失。最近,研究者已先后建立了基于靶向RNA重组技术、细菌人工染色体(BAC)载体系统和体外连接技术的PEDV反向遗传学操作技术,为PEDV编码的蛋白质的功能、致病机制以及新型疫苗的研发开辟了新的思路。本文对PEDV反向遗传操作技术的研究进展及其在PEDV胰酶依赖性、S蛋白和ORF3蛋白的功能以及新一代疫苗研制等方面的应用现状进行了综述与展望。
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| 关 键 词： | 猪流行性腹泻病毒，反向遗传学，胰酶，S蛋白，ORF3蛋白，细胞适应性，疫苗 |
| 收稿时间： | 2016-06-26 |
Advances in reverse genetics to treat porcine epidemic diarrhea virus |
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| Ruisong Yu,Shijuan Dong,Fusheng Si,Fengying Jiang,Chunfang Xie,Bingqing Chen,Li Yu and Zhen Li. Advances in reverse genetics to treat porcine epidemic diarrhea virus[J]. Chinese journal of biotechnology, 2017, 33(2): 205-216 |
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| Authors: | Ruisong Yu Shijuan Dong Fusheng Si Fengying Jiang Chunfang Xie Bingqing Chen Li Yu Zhen Li |
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| Affiliation: | 1 Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 2 Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201106, China,1 Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 2 Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201106, China,1 Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China,1 Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 2 Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201106, China,1 Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 2 Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201106, China,1 Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China,1 Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China and 1 Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 2 Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201106, China |
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| Abstract: | Porcine epidemic diarrhea virus (PEDV) is one of the major etiologies responsible for the acute, highly contagious disease in the digestive tract of pigs, especially neonatal piglets. Since PEDV was first identified in Europe in the late 1970s, it has resulted in significant economic losses in many Asian swine-raising countries, including China. Recently, reverse genetics techniques including targeted RNA recombination, bacteria artificial chromosome system and in vitro ligation have been successfully used to manipulate the genome of PEDV, which providing new strategies for the clear delineation of the functions of the viral proteins, the mechanisms behind PEDV pathogenesis and the design of novel vaccines against PEDV. Here, we review the progresses of different reverse genetics platforms developed for PEDV and their applications, covering the roles of trypsin in PEDV propagation, functions of S and ORF3 protein and the development of next generation PED vaccines, and the perspectives of reverse genetics for PEDV. |
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| Keywords: | porcine epidemic diarrhea virus (PEDV) reverse genetics trypsin S protein ORF3 protein cell adaption vaccine |
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