A simple method for the fluorescence analysis of nucleic acid-dye complexes in cytological preparations |
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| Authors: | A. Villanueva J. C. Stockert R. Armas-Portela |
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| Affiliation: | (1) Departamento de Citología e Histología, Facultad de Ciencias, Universidad Autonoma de Madrid, 34 Madrid, Spain |
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| Abstract: | Summary This communication describes a simple method for recording fluorescence emission spectra of cytological preparations using a conventional fluorescence spectrophotometer. The emission characteristics of  in situ complexes between some basic fluorochromes (DAPI, 33258 Hoechst, acridine orange, pyronin Y, and ethidium bromide) and nucleic acid containing structures from smears of chicken blood and Ehrlich tumor cells (chromatin, basophilic cytoplasm) are briefly described. |
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