Effect of rat salivary glands extracts on the proliferation of cultured skin cells - a wound healing model |
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Authors: | Nili?Grossman Li-At?Binyamin Email author" target="_blank">Lipa?BodnerEmail author |
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Institution: | (1) Skin Bank and Investigative Dermatology Laboratory, Soroka University Medical Center, Beer-Sheva, Israel;(2) Department of Microbiology and Immunology, Ben-Gurion Cancer Research Center, Beer-Sheva, Israel;(3) Department of Oral and Maxillofacial Surgery, Soroka University Medical Center, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer-Sheva, Israel |
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Abstract: | Objective: Salivary gland secretions play an important role in promotion of wound healing. The healing of intra- or extra-oral wounds
is delayed in desalivated rats. However, the specific role of each salivary gland in promoting wound healing is unknown. This
study was aimed to investigate the effect of crude extracts of rat salivary glands on a simplified in vitro wound healing model. Design/methods: Cultured human keratinocytes (HaCat) and murine fibroblasts (3T3) were subjected to 48 h serum starvation, and were later
activated by extracts of rat salivary glands, 1–10 μg protein/ml of each gland. The resultant cellular metabolic activity
of the activated cells was determined 24 h later, measuring reduction of XTT by mitochondrial enzymes, and calculated relatively
to positive controls optimal supplementation of 10% fetal calf serum (FCS)], and negative controls (starved non-supplemented
cells). Results: The relative stimulatory effect of parotid (P) extract on the cells was significantly lower than either submandibular (SM)
or sublingual (SL) extracts. Under the assumption that physiologically, the cells are exposed to the combined effect of saliva
secreted from all the glands, different combinations of the extracts were presented to the cells. The relative stimulation
was maximal following treatment with the three glands extracts (P + SM + SL) and exceeded the effect of 10% FCS. Conclusion: The results suggest that each salivary gland has a specific effect on wound healing and the combination of the three extracts
has an additive effect but no the sum of all individual glands. This model might be useful to study the wound healing effect
of salivary glands.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Cultured cells Salivary glands Wound healing |
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