An antibody-lectin sandwich assay for quantifying protein glycoforms |
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Authors: | Fionnuala T. Lundy G. Brian Wisdom |
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Affiliation: | (1) School of Biology and Biochemistry, The Queen’ University, Medical Biology Centre, BT9 BL Belfast, UK-Northern Ireland |
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Abstract: | We describe an antibody-lectin sandwich assay for quantitation of glycoforms of proteins. The assay uses deglycosylated IgG antibody immobilized on a microtiter plate to capture the protein of interest from the sample. The particular glycoform is then identified by reaction with biotin-labeled lectin, which is measured using streptavidin/alkaline phosphatase. The assay can be adapted to quantitate any protein’s glycoforms by simply substituting the antibody and lectin with specific alternatives, |
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Keywords: | Glycoprotein glycoform lectin antibody assay |
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