Immunolocalization of lipoxygenase in pea (Pisum sativum L.) carpels

Summary Polyclonal antibodies against a part of pea (Pisum sativum L.) LOXG protein have been raised to study the pattern of distribution of related lipoxygenases in pea carpels. The antiserum recognized three lipoxygenase polypeptides in carpels. One of them became undetectable 24 hours after fruit development induction, suggesting that it may correspond to the protein derived from loxg cDNA. Immunolocalization experiments showed that lipoxygenase protein was present only in pod tissues: it was abundant in the mesocarp and, from the day of anthesis, in the endocarp layers. Lipoxygenase distribution is regulated throughout development. The association of lipoxygenase with cells in which processes of expansion and growth will potentially take place support a role in pod growth and development.Abbreviations DTT dithiothreitol - EDTA ethylenediaminetetraacetic acid - IgG immunoglobulin G - GA₃ gibberellic acid - LOX lipoxygenase - PAGE polyacrylamide gel - PVDF polyvinylidene difluoride - SDS sodium dodecyl sulfate - Tris 2-amino-2-hydroxymethyl-1,3-propanediol