One-step isolation of plant DNA suitable for PCR amplification |
| |
| Authors: | Karen Burr Ross Harper Adrian Linacre |
| |
| Institution: | (1) Medical Research Council Toxicology Unit, University of Leicester, UK;(2) International Forensic Research Institute, Florida International University, 33199 Miami, Florida;(3) Forensic Science Unit, Pure and Applied Chemistry, University of Strathclyde, UK;(4) Present address: Forensic Science Unit, Department of Pure & Applied Chemistry, University of Strathclyde, 204 George Street, G1 1XW Glasgow, UK |
| |
| Abstract: | We report a one-step extraction technique for the isolation of plant DNA, DNA suitable for amplification by PCR can be produced
from leaf material smaller than 0.3 mm2 in less than 20 min, with no tube changes. The method was tested on several plant specA00AK020ies. The described method was
found to extract DNA that could be amplified without any further purification or treatment. The isolated DNA was amplified
using a universal chloroplast primer set. The method was validated by comparing size of PCR products generated by the novel
method to PCR products generated using standard DNA isolation techniques. |
| |
| Keywords: | chloroplast markers DNA isolation grasses RAPD |
| 本文献已被 SpringerLink 等数据库收录！ |
|
