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Interaction between single molecules of Mac-1 and ICAM-1 in living cells: an atomic force microscopy study
Authors:Yang Huayan  Yu Junping  Fu Guo  Shi Xiaoli  Xiao Lin  Chen Yizhang  Fang Xiaohong  He Cheng
Affiliation:Department of Neurobiology, Institute of Neuroscience, Second Military Medical University, Shanghai, China.
Abstract:The interaction between integrin macrophage differentiation antigen associated with complement three receptor function (Mac-1) and intercellular adhesion molecule-1 (ICAM-1), which is controlled tightly by the ligand-binding activity of Mac-1, is central to the regulation of neutrophil adhesion in host defense. Several "inside-out" signals and extracellular metal ions or antibodies have been found to activate Mac-1, resulting in an increased adhesiveness of Mac-1 to its ligands. However, the molecular basis for Mac-1 activation is not well understood yet. In this work, we have carried out a single-molecule study of Mac-1/ICAM-1 interaction force in living cells by atomic force microscopy (AFM). Our results showed that the binding probability and adhesion force of Mac-1 with ICAM-1 increased upon Mac-1 activation. Moreover, by comparing the dynamic force spectra of different Mac-1 mutants, we expected that Mac-1 activation is governed by the downward movement of its alpha7 helix.
Keywords:Mac-1   ICAM-1   AFM   Single molecule   Dynamic force spectroscopy   Binding probability   Rupture force   Activation   Living cell
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