Trypanosoma cruzi: in vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg) |
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Authors: | Güida María C Esteva Mónica I Camino Alejandra Flawiá Mirtha M Torres Héctor N Paveto Cristina |
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Affiliation: | Instituto de Investigaciones en Ingeniería Genética y Biología Molecular y Facultad de Ciencias Exactas y Naturales (CONICET-UBA), 1428 Buenos Aires, Argentina. mcguida@dna.uba.ar |
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Abstract: | The trypanocidal activity of catechins on Trypanosoma cruzi bloodstream trypomastigotes has been previously reported. Herein, we present the effect of epigallocatechin gallate (EGCg) on parasitemia and survival in a murine model of acute Chagas' disease as well as on the epimastigote form of the parasite. Upon intraperitoneal administration of daily doses of 0.8 mg/kg/day of EGCg for 45 days, mice survival rates increased from 11% to 60%, while parasitemia diminished to 50%. No side effects were observed in EGCg-treated animals. Fifty percent inhibition of epimastigotes growth was achieved with 311 microM EGCg 120 h after drug addition. No lysis, total culture growth inhibition or morphological changes were observed upon addition of 1-3mM EGCg at 24 h. This treatment also produced oligosomal fragmentation of epimastigotes DNA, suggesting a programmed cell death (PCD)-like process. All these findings point out EGCg as a potential new lead compound for chemotherapy of Chagas' disease. |
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Keywords: | Epigallocatechin gallate Trypanocide Chagas’ disease PCD, Programmed cell death |
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