Sequence analysis of rDNA intergenic spacer (IGS) of <Emphasis Type="Italic">Porphyra haitanensis</Emphasis> |
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Authors: | Yanyan Li Songdong Shen Lihong He Pu Xu Shan Lu |
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Institution: | (1) School of Medicine and Life Sciences, Medical College of Soochow University, Suzhou, 215123, China;(2) Department of Biology and Food Engineering, Changshu Institute of Technology, Suzhou, 215500, China;(3) School of Life Sciences, Nanjing University, Nanjing, 210093, China; |
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Abstract: | The intergenic spacer region (IGS) has been used for the first time to analyze the genetic variability of Porphyra haitanensis from different areas. In order to determine that whether the IGS sequences could be used for classification and identification
in intraspecies of Porphyra, the partial IGS sequences of cultivated strains of P. haitanensis (isolated from Putian-Fujian Province, Shantou-Guangdong Province and Ningbo-Zhejiang Province), were amplified, sequenced
and analyzed. The sequence analysis indicated that the partial IGS sequences from the three stains were the external transcribed
spacers (ETS) of 3′ end of the IGS gene. In the three stains, the length of IGS sequences ranged from 1,085 to 1,100 bp and
the G + C content varied from 50.88% to 51.27%. There were 55 variable sites which occupied approximately 5% of the ETS sequences.
Similarity analysis and multisequencing alignment of sequences indicated that the partial IGS sequences of the three stains
of P. haitanensis had notable variabilities. Therefore, the IGS sequence could be used as the critical genetic marker in intraspecies of P. haitanensis. Furthermore, IGS sequence analysis will be a powerful tool for genetic diversity and classification in intraspecies of other
Porphyra species. |
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