Double-Staining Method for Differentiation of Morphological Changes and Membrane Integrity of Campylobacter coli Cells |
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| Authors: | Jose L Alonso Salvatore Mascellaro Yolanda Moreno María A Ferrús and Javier Hernndez |
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| Institution: | Jose L. Alonso, Salvatore Mascellaro, Yolanda Moreno, María A. Ferrús, and Javier Hernández |
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| Abstract: | We developed a double-staining procedure involving NanoOrange dye (Molecular Probes, Eugene, Oreg.) and membrane integrity stains (LIVE/DEAD BacLight kit; Molecular Probes) to show the morphological and membrane integrity changes of Campylobacter coli cells during growth. The conversion from a spiral to a coccoid morphology via intermediary forms and the membrane integrity changes of the C. coli cells can be detected with the double-staining procedure. Our data indicate that young or actively growing cells are mainly spiral shaped (green-stained cells), but older cells undergo a degenerative change to coccoid forms (red-stained cells). Club-shaped transition cell forms were observed with NanoOrange stain. Chlorinated drinking water affected the viability but not the morphology of C. coli cells. |
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