A novel pathway for rapid shoot regeneration from the proximal zone of the inpocotyl of melon (Cucumis melo L.) |
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Authors: | S Curuk C Elman E Schlarman O Sagee I Shomer S Cetiner D J Gray V Gaba |
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Institution: | (1) Department of Virology, ARO Volcani Center, POB 6, 50250 Bet Dagan, Israel;(2) Department of Citriculture, ARO Volcani Center, POB 6, 50250 Bet Dagan, Israel;(3) Department of Food Science, ARO Volcani Center, POB 6, 50250 Bet Dagan, Israel;(4) Laboratory of Plant Biotechnology, University of Cukorova, Adana, Turkey;(5) Mid-Florida Research & Education Center, IFAS, University of Florida, Binion Road, 2725 Apopka, FL, 32703-8504;(6) Present address: Faculty of Agriculture, Department of Horticulture, University of Mustafa Kemal, 31034 Antakya-HATAY, Turkey;(7) Present address: Faculty of Engineering and Natural Sciences, Sabanci University, 31474 Tuzla. Istanbul, Turkey |
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Abstract: | Summary Hypocotyl explants of melon (Cucumis melo L.) are capable of regenerating multiple shoots on Murashige and Skoog (1962) medium, augmented with 4.4 μM benzylademne. Regeneration from the hypocotyl is much more rapid than the more commonly reported regeneration from cotyledonary
explants, producing shoots within 2 wk compared to more than a month required for cotyledon explants. The rapid regeneration
response depends on the presence of a fragment of the cotyledon remaining attached to the hypocotyl. Controls were performed
to ensure that the regeneration was not due to the presence of the shoot apical bud of the melon seedling after explant production.
Scanning electron microscopy revealed that microsurgery to remove the apical bud left no excess bud material. Regeneration
from the proximal part of the hypocotyl was due to production of a new shoot apical meristem, observed by histology. The apical
meristem can be produced before leaf primordia in regeneration from the hypocotyl, in contrast to the regeneration process
from the melon cotyledon. |
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Keywords: | organogenesis bud regeneration shoot apex cotyledon cucurbits |
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