DIURNAL VARIATION IN PROLIFERATIVE COMPARTMENTS AND THEIR RELATION TO CRYPTOGENIC CELLS IN THE MOUSE COLON

The depth of the crypts in mouse descending colon varied diurnally, between twenty-six cells at 24.00 hours and thirty-eight cells at 12.00 hours. Cell loss from the colon was greatest immediately before the maximum faeces production, at the beginning of the dark period. The labelling index of the colon also changed, from 9% at 20.00 hours to 16% at 12.00 hours. The greatest variation in labelling index occurred at the top of the zone of proliferative cells, between the ninth and eighteenth cell position up the crypt. In this region a synchronized cohort of about forty cells apparently entered S phase once a day. Although the length of the proliferative zone doubled at 12.00 hours, that of the non-proliferative zone remained fairly constant all day. The number of cryptogenic cells per crypt was estimated by comparing single and split-dose X-ray survival curves. This gave a mean value of two cryptogenic cells per crypt. Crypts rarely regenerated from the base after irradiation. The cryptogenic cells probably lay between cell positions Nos 9 and 18 up the crypt and probably did not function as stem cells in the normal crypt.