HPLC法测定牙膏中柚皮苷的含量

建立了高效液相色谱测定牙膏中柚皮苷含量的方法。采用的色谱条件:Hypersil BDS C18色谱柱(250 mm×4.6 mm,5μm);柱温为40℃;以水(A相)和乙腈(B相),梯度洗脱程序为:0～15 min,10%～100%B;流速为1.0 mL/min;检测波长为283 nm;进样量为20μL。结果表明,柚皮苷的质量浓度在14.55～116.40μg/mL范围内与峰面积呈良好的线性关系(r=0.9999),平均加标回收率为97.56%。该方法稳定、准确,重现性好,可作为牙膏中柚皮苷的含量测定和质量控制方法。

Determination of naringin in toothpaste by high performance liquid chromatography

A high performance liquid chromatographic method was developed for determining the content of Naringin in toothpaste.The chromatographic analysis was carried out on a Hypersil BDS C18 column(250 mm×4.6 mm,5μm).The mobile phase was water(A)and acetonitrile(B)with gradient elution(0～15 min,10%～100%B).The flow rate was maintained at 1.0 mL/min;The detection wavelength was set at 283 nm and the column temperature was controlled at 40℃,The sample injection volume was 20μL.The results showed that the mass concentration of Naringin with good linearity vs.the peak area within ranges of 14.55～116.40μg/mL,r=0.9999.The average recovery was 97.56%.The method was proved to be stable,reproducible and precise,It could be used for determination and quality control of naringin in toothpaste.