首页 | 本学科首页   官方微博 | 高级检索  
     

HPLC法测定牙膏中柚皮苷的含量
引用本文:覃青云,卢凯玲. HPLC法测定牙膏中柚皮苷的含量[J]. 广西植物, 2009, 29(5): 707-709
作者姓名:覃青云  卢凯玲
作者单位:柳州两面针股份有限公司,广西,柳州,545001;柳州两面针股份有限公司,广西,柳州,545001
摘    要:建立了高效液相色谱测定牙膏中柚皮苷含量的方法。采用的色谱条件:Hypersil BDS C18色谱柱(250 mm×4.6 mm,5μm);柱温为40℃;以水(A相)和乙腈(B相),梯度洗脱程序为:0~15 min,10%~100%B;流速为1.0 mL/min;检测波长为283 nm;进样量为20μL。结果表明,柚皮苷的质量浓度在14.55~116.40μg/mL范围内与峰面积呈良好的线性关系(r=0.9999),平均加标回收率为97.56%。该方法稳定、准确,重现性好,可作为牙膏中柚皮苷的含量测定和质量控制方法。

关 键 词:HPLC法  牙膏  柚皮苷

Determination of naringin in toothpaste by high performance liquid chromatography
QIN Qing-Yun,LU Kai-Ling. Determination of naringin in toothpaste by high performance liquid chromatography[J]. Guihaia, 2009, 29(5): 707-709
Authors:QIN Qing-Yun  LU Kai-Ling
Affiliation:Liuzhou Liangmianzhen Co.Ltd, Liuzhoui 545001, China
Abstract:A high performance liquid chromatographic method was developed for determining the content of Naringin in toothpaste.The chromatographic analysis was carried out on a Hypersil BDS C18 column(250 mm×4.6 mm,5μm).The mobile phase was water(A)and acetonitrile(B)with gradient elution(0~15 min,10%~100%B).The flow rate was maintained at 1.0 mL/min;The detection wavelength was set at 283 nm and the column temperature was controlled at 40℃,The sample injection volume was 20μL.The results showed that the mass concentration of Naringin with good linearity vs.the peak area within ranges of 14.55~116.40μg/mL,r=0.9999.The average recovery was 97.56%.The method was proved to be stable,reproducible and precise,It could be used for determination and quality control of naringin in toothpaste.
Keywords:HPLC  toothpaste  Naringin
本文献已被 万方数据 等数据库收录!
点击此处可从《广西植物》浏览原始摘要信息
点击此处可从《广西植物》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号