| 长期保存丝核菌菌种的一种方法 |
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| 引用本文： | 杨根华,王雪媛,陈海如,生越明.长期保存丝核菌菌种的一种方法[J].菌物学报,2002,21(1):140. |
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| 作者姓名： | 杨根华 王雪媛 陈海如 生越明 |
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| 作者单位： | 1. 云南农业大学植物病理重点实验室,昆明650201
2. 北海道大学农学部,札幌,日本 |
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| 基金项目： | Supported by the natural Science Foundation of Yunnan Province (云南省自然科学基金资助) |
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| 摘 要： | Cultures of Rhizoctonia spp. maintained on agar media at 15℃ should be transferred every 4-5 months to ensure viability. This procedure could consume an excessive amount of time, and isolates that are transferred frequently may undergo genetic changes resulting in reduction in virulence. Therefore, methods for long-term storage (>6 months) are required. 1 MATERIALS AND METHODS More than 130 strains of Rhizoctonia spp. were isolated from the soil and the infected plant tissues in Yunn…
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| 修稿时间： | 2001年8月20日 |
A METHOD FOR LONG-TERM STORAGE OF RHIZOCTONIA SPP. |
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| Abstract: | Cultures of Rhizoctonia spp. maintained on agar media at 15℃ should be transferred every 4-5 months to ensure viability. This procedure could consume an excessive amount of time, and isolates that are transferred frequently may undergo genetic changes resulting in reduction in virulence. Therefore, methods for long-term storage (>6 months) are required. |
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