紫膜碎片的电二色性研究 |
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引用本文: | 王敖金,谈曼琪,孙纹琦,孙永泰. 紫膜碎片的电二色性研究[J]. 生物物理学报, 1990, 6(4): 472-475 |
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作者姓名: | 王敖金 谈曼琪 孙纹琦 孙永泰 |
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作者单位: | 中国科学院生物物理研究所(王敖金,谈曼琪,孙纹琦),中国科学院生物物理研究所(孙永泰) |
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基金项目: | 中国科学院“七五”重大项目基金资助 |
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摘 要: | 悬浮在水中的嗜盐菌紫膜碎片,在外电场作用下产主定向排列.在20℃时,568nm的电二色性研究表明:外加电场为2kV/m时取向程度可达60%以上;大于5.5kV/m时,取向作用趋于饱和状态;饱和时简约电二色性为-0.437左右,视黄醛生色团的跃迁矩方向与电偶极矩方向形成60.9°夹角;紫膜的永久偶极短为9.2×10~(-24)C、M,剩余电极化率为3.0×10~(-27)m~2;紫膜的旋转扩散常数为0.53秒~(-1).曲线拟合分析表明,感应偶极对紫膜碎片的定向的贡献应予考虑.本文对紫膜碎片的定向机理进行了讨论.
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关 键 词: | 紫膜 电二色性 |
ELECTRICALLY LINEAR DICHROISM STLDEES OF PURPLE MEMBRANE FRAGMENTS |
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Abstract: | Purple membrane (PM) fragments from Halobacterium halobium R1M1 were oriented by a static electric field in a water suspension. It was found by electrically linear dichroism at 20 C that the orientation degree is up to 60% when an electric field of 2kV/m is applied, and the orientation is tend to saturation when the field is more than 5.5kV/m; that the PM has a permanent electric dipole moment of 9.2 × 10-24C. m, and their electrically excess polarizablity of 3.0 × 10-27F. m2, the orientation of the retinal transition moment relative to the direction of the electric dipole moment is 60.9°, and the PM rotational diffusion constant D=0.53S-1.The curve fitting analysis showed that, the contribution of the induced electric dipole to the orientation of the PM fragments should be considered. |
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