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Localization of Some Enzymes of β-oxidation of Fatty Acids in the Peroxisomes of Tetrahymena*
Authors:J J BLUM
Abstract:Mitochondria and peroxisomes were prepared from homogenates of Tetrahymena pyriformis by sedimentation through sucrose gradients. Catalase and isocitrate lyase served as peroxisomal markers; lactic dehydrogenase and glutamic dehydrogenase as mitochondrial markers. Acetyl-CoA synthetase, octanoyl-CoA synthetase, palmitoyl-CoA synthetase, 3-β hydroxyacyl CoA dehydrogenase, and thiolase activities were found in both the peroxisomes and the mitochondria. It is suggested that β-oxidation of fatty acids accurs in both organelles in Tetrahymena.
Keywords:Tetrahymena  peroxisomes  fatty acid activating enzymes  β  -oxidation
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