Introductory study of an oocyte membrane protein that specifically binds vitellogenin in the crayfish Orconectes limosus |
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Authors: | P JUGAN F VAN HERP |
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Institution: | Zoological Laboratory, Faculty of Sciences , Catholic University , Toernooiveld, 6525, ED Nijmegen, The Netherlands |
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Abstract: | Summary After solubilization of membranes from vitellogenic oocytes of the crayfish Orconectes limosus, a component with an apparent molecular weight between 28 and 30 kDa that specifically binds vitellogenin, could be identified by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and by electroblotting. Because this component was pronase sensitive, we assume that we are dealing with a protein. A polyclonal rabbit antiserum was produced against this component and its tissue specificity was verified. |
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Keywords: | membrane protein vitellogenic oocytes vitellogenin crayfish Orconectes limosus |
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