Nuclear binding sites for juvenile hormone III in the male accessory reproductive glands of Melanoplus sanguinipes |
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Authors: | SHEIKH M. ISMAIL CEDRIC GILLOTT |
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Affiliation: | Department of Biology , University of Saskatchewan , 112 Science Place, Saskatoon, Saskatchewan, S7N 5E2, Canada Phone: (306) 966-4401 Fax: (306) 966-4401 |
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Abstract: | Summary We have identified a potential nuclear juvenile hormone (JH) receptor in the long hyaline tubules (LHT), part of the male accessory reproductive gland (MARG) of M. sanguinipes. The MARG was incubated in vitro with [3H]JH III, and the distribution of the [3H]JH III among the cellular fractions of the LHT was determined. Some 37±4% of the radioactivity was associated with the crude nuclear pellet, while the cytosolic, microsomal and mitochondrial fractions contained 30±3%, 23±2% and 10±1%, respectively. The bound JH III was measured in nuclear extracts of LHT from males up to 15 days post-eclosion. These results revealed that JH binding increased in an age-dependent manner up to day 7, then levelled off to day 12, to increase again on day 14. The nuclear-binding component in the LHT had a very strong affinity for JH III, with a KD value of 0.8 nM. Our observations are considered in relation to the potential site and mode of action of JH. |
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Keywords: | Juvenile hormone Melanoplus sanguinipes male accessory reproductive gland nuclear binding sites |
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