Quantitative determination of 6-keto-PGF1α released by rat aorta: Comparison of mass fragmentography and high resolution gas chromatography with electron capture detection |
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| Authors: | C Chiabrando A Noseda MA Noè R Fanelli |
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| Institution: | Laboratory of Environmental Pharmacology and Toxicology, “Mario Negri” Institute for Pharmacological Research, Via Eritrea, 62 - 20157 Milan, Italy |
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| Abstract: | Mass fragmentography (MF) and high resolution gas chromatography with electron capture detection (HRGC-ECD) were used for measuring 6-keto-PGF1α, the stable hydrolysis product of prostacylin (PGI2) released by fresh rings of rat aorta, incubated in the absence of the precursors arachidonic acid or prostaglandin endoperoxide (PGH2). The incubation medium was acidified, extracted, chromatographed on silicic acid column and derivatized. Comparable results were obtained analyzing each sample by MF and HRGC-ECD. Both methods proved to be suitable in terms of sensitivity and specificity for the measurement of 6-keto-PGF1α produced by individual rat aortae. |
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