Pluripotent Haemopoietic Stem Cell Entry Into Dna Synthesis In Vitro After Stimulation By Ara-C Treatment In Vivo |
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| Authors: | H. Izumi M. Guigon E. Frindel |
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| Affiliation: | Unitéde recherches sur la cinétique cellulaire INSERM U-250, Institut Gustave-Roussy 94805 Villejuif France |
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| Abstract: | Abstract. Non-cycling pluripotent bone marrow stem cells (CFU-S), taken 3 hrs after injection of 20 mg of Ara-C, have been shown to enter DNA synthesis at 1 to 3 hr after being cultured in α-medium. This phenomenon was observed when bone marrow was incubated as a plug, but not when incubated as a cell suspension in the present experimental conditions.
These results suggest that a medullary structure is necessary in order to observe this effect and/or that accessory cells are destroyed during the process of single cell preparation. |
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