Effect of penicillin G on the electroporation of Rhodococcus rhodochrous CF222 |
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| Authors: | M Sunairi N Iwabuchi K Murakami F Watanabe Y Ogawa H Murooka M Nakajima |
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| Institution: | Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Agriculture and Veterinary Medicine, Nihon University, Fujisawa, Kanagawa, Japan |
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| Abstract: | M. SUNAIRI, N. IWABUCHI, K. MURAKAMI, F. WATANABE, Y. OGAWA, H. MUROOKA AND M. NAKAJIMA. 1996. Suitable conditions for the introduction of bacteriophage DNA into cells of Rhodococcus rhodochrous CF222 by electroporation were established, and penicillin G was found to enhance the transfection frequency. When conditions optimal for the parental strain were applied to its colony-morphological mutants, different transfection frequencies were observed. Penicillin G enhanced the transfection frequency of smooth and mucoidal mutants but not of rough mutants. |
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