Partial characterization of a cell-free hemolytic factor produced by Helicobacter pylori |
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Authors: | BL Wetherall PJ McDonald AM Johnson |
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Institution: | Department of Microbiology and Infectious Diseases, School of Medicine, Flinders Medical Center, Bedford Park, South Australia 5042, Australia;Flinders University of South Australia Flinders Medical Centre, Bedford Park, South Australia 5042, Australia |
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Abstract: | Abstract Maximum cell-free hemolytic activity of Helicobacter pylori cultured in broth containing 10% horse serum occurred only after the stationary phase of growth was reached, unlike many hemolysins produced by Gram-negative bacteria which are active during exponential growth. This characteristic of the H. pylori hemolytic factor suggested that it might also possess protease activity. However, because no evidence of albumin degradation was found, the hemolysis by cell-free concentrates of H. pylori appears to be due to a unique factor derived from the organism. Because variable hemolysis results were obtained with culture broths lacking albumin or serum, these proteins may act as carriers or stabilizers of the putative hemolysin. |
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Keywords: | Helicobacter pylori Hemolytic activity |
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